Lentinula edodes Cultured Extract and Rouxiella badensis subsp. acadiensis (Canan SV-53) Intake Alleviates Immune Deregulation and Inflammation by Modulating Signaling Pathways and Epigenetic Mechanisms

Puberty is a critical developmental period of life characterized by marked physiological changes, including changes in the immune system and gut microbiota development. Exposure to inflammation induced stressors during puberty has been found stimulate central lead disturbance at distant sites from gut; however, its enduring effects on immunity are not well explored. Therefore, this study, we used pubertal lipopolysaccharides (LPS)-induced mouse model mimic exposure dysbiosis. We hypothesized that LPS-induced may cause long-term dysfunction dysregulation inflammatory signaling epigenetic while prebiotic/probiotic intake mitigate deregulation later life. To end, four-week-old female Balb/c mice were fed prebiotics/probiotics exposed LPS window. better decipher acute immunoprotective biotic intake, addressed effect treatment interleukin (IL)-17 related-cytokines pathways. In addition, alterations, microRNA (miRNA) expression DNA methylation, studied. Our results revealed significant selected cytokines, proteins, miRNAs involved key pathways related IL-17 production function, IL-17A F, IL-6, IL-1β, transforming growth factor-β (TGF-β), signal transducer activator transcription-3 (STAT3), p-STAT3, forkhead box O1 (FOXO1), miR-145 small intestine adult challenged with puberty. contrast, dietary interventions mitigated lasting adverse partly through mechanisms. A methylation analysis demonstrated might be linked differentially methylated genes, Lpb, Rorc, Runx1, Il17ra, Rac1, Ccl5, Il10, Th17 cell differentiation signaling. prebiotic administration prevented mice. Together, these indicate following healthy diet rich prebiotics probiotics an optimal strategy for programming function windows controlling